Nucleotide sequence of rat adipose hormone sensitive lipase cDNA
نویسندگان
چکیده
منابع مشابه
Positional specificity of hormone-sensitive lipase from rat adipose tissue.
Hormone-sensitive lipase, purified from rat adipose tissue (Fredrikson, G., Strålfors, P., Nilsson, N. O., and Belfrage, P. (1981) J. Biol. Chem. 256, 6311-6320), has been incubated with tri-, di-, and monooleoyl[3H]glycerol, and the acylglycerol reaction products were isolated by thin layer chromatography on silicic acid, impregnated with boric acid. Trioleoylglycerol was hydrolyzed with the i...
متن کاملHormone-sensitive Lipase and Monoglyceride Lipase Activities in Adipose Tissue.
Several workers (l-3) have reported that lipase activity assayed in homogenates of adipose tissue is increased by exposure of the tissue to epinephrine or to adrenocorticotropic hormone prior to homogenization. In general, the magnitude of the increases observed has been small compared to the large effects of these hormones on glycerol format,ion by the intact tissae. In studies of whole homoge...
متن کاملRapid communication: nucleotide sequence of the ovine lipoprotein lipase cDNA.
Name of the Sequence. Ovine lipoprotein lipase cDNA. Genus and Species. Ovis aries. Origin of Clones. Because almost the whole 3′-untranslated region (3′UTR) was found to be lacking from the ovine lipoprotein lipase (LPL) cDNA sequence (1,656 bp including the 5′UTR, the coding sequence, and the first 44 bp of the 3′UTR) published by Edwards et al. (1993), we have undertaken to characterize this...
متن کاملStudies on the hormone-sensitive lipase of adipose tissue.
Sucrose gradient centrifugation has been used to examine the triglyceride lipases present in extracts of rat epididymal adipose tissue. The aqueous infranatant recovered between the pellet and fat cake of tissue homogenates which had been centrifuged at 40,000 g was shown to contain two types of triglyceride lipase activity. One of these appears in the 15s region and has been identified as the ...
متن کاملRapid assay for hormone-sensitive lipase activity of adipose tissue.
A highly specific and rapid assay for hormone-sensitive lipase activity of rat adipose tissue is described. The method employs emulsified 2,3-di-O-oleyl-[9,10-(3)H(2)]oleoyl glycerol as a substrate; it is very sensitive and is suitable for serial sampling.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1988
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/16.20.9879